Figure 3

Intracellular survival of E. coli K1 by unstimulated, palmitoylethanolamide (PEA)- and lipopolysaccharide (LPS)-stimulated microglial cells (a) and macrophages (b). Intracellular survival was expressed as the number of bacteria (median) recovered at the different time points (n ≥ 9 wells/group from two independent experiments (a), n ≥ 12 wells/group from two independent experiments (b)). For statistical comparisons the median number of bacteria at zero hours was calculated. Then, the number of bacteria at five hours of each individual well was subtracted from the median number at zero hours, and these differences were compared by Kruskall-Wallis Test followed by Dunn’s correction for repeated testing (***P < 0.001, *P < 0.05).