Figure 2

IL-1β attracts migration of cortical neurons in vitro . (A) Schematic diagram of transwell assay. (B, C) Photomicrographs of stained cells that crossed the 8 μm pore after 20 hours of migration in the absence (B) or presence (C) of IL-1β (1 ng/mL) in the lower chamber. Scale bar, 100 μm. (D) Number of migrated neurons normalized to the value of the parallel control when IL-1β was added to the lower chamber at concentrations of 0.1 ng/mL, 1 ng/mL and 10 ng/mL, respectively. (E) Number of migrated neurons normalized to the value of the parallel control when IL-1β was added to the upper chamber, lower chamber and both, respectively. (F) Number of migrated neurons normalized to the value of the parallel control when IL-1RA at different concentrations was added to the upper and lower chambers before addition of IL-1β in the lower chamber. **P <0.01 versus control group.