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Figure 3 | Journal of Neuroinflammation

Figure 3

From: Interleukin-1 beta guides the migration of cortical neurons

Figure 3

IL- 1β guides axonal growth cone turning. (A-C) Microscopic images of the growth cone of a cerebellar granule cell at the beginning and the end of a one-hour exposure to a gradient of PBS, BDNF (20 μg/ml in the pipette) or IL-1β (1 μg/ml in the pipette). Superimposed traces (right) depict the trajectory of neurite extension during the one-hour period for all neurons examined. The origin is the center of the growth cone at the onset of the experiment, and the original direction of growth was vertical. The arrow indicates the direction of the gradient. Scale bar, 50 μm. (D) The distribution of turning angles. For each experimental condition, the angular positions of all growth cones at the end of a one-hour exposure to a gradient of PBS, BDNF or IL-1β are shown in the cumulative frequency plot. The percentage value refers to the percentage of growth cones with angular position ≤ a given angular value. (E) The average turning angle during the one-hour assay for all neurons examined. (F) The neurite extension rate during the one-hour assay for all neurons examined. **P <0.01 versus PBS group. BDNF, brain-derived neurotrophic factor.

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