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Figure 7 | Journal of Neuroinflammation

Figure 7

From: Interleukin-1 beta guides the migration of cortical neurons

Figure 7

Down-regulation of IL-1R1 did not affect the proliferation and radial glia scaffold of neural progenitor cells. (A) Representative images showing BrdU incorporation in control (scramble sequence) and IL-1R1-i1-transfected brains. BrdU was injected intraperitoneally into pregnant rats 24 hours after IUE, and embryos were harvested after another 24 hours. Brain sections were stained with anti-BrdU (red) and anti-GFP (green) antibodies. (B) Images of radial glia scaffold counterstained with anti-Nestin antibody in P0 cortices electroporated with scramble or IL-1R1-i1. The selected regions in the left panels are shown in high magnification at right. Transfection with IL-1R1-i1 did not cause obvious changes in the morphology of radial glial scaffold in cortices. (C) Histograms showing the percentage of BrdU+/GFP + cells in total transfected cells (GFP+) (BrdU index) in the two groups. Numbers in brackets are numbers of brains analyzed in each group. No significant differences between scramble and IL-1R1-i1 groups were found (P = 0.22, Student’s t test). Scale bar, 100 μm. BrdU, bromodeoxuridine; P, postnatal day.

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