Figure 12
From: Glimepiride reduces CD14 expression and cytokine secretion from macrophages
PrP82-146 causes the translocation of Toll-like receptor (TLR)-4 into rafts. (A) The % of TLR-4 in DRMs derived from RAW 264 cells treated with PrP82-146 (●) or PrP82-146scrambled (○). Values are means ± SD from triplicate experiments performed 3 times, n = 9. (B) The % TLR-4 in DRMs derived from RAW 264 cells treated with control medium (□) or 50 μM Aβ1–42, Aβ42–1, αSN or βSN as shown (■). Values are means ± SD from triplicate experiments performed 3 times, n = 9. *TLR-4 significantly greater than those of control cells. (C) RAW 264 cells were incubated with PrP82-146 (○), Aβ1–42 (●) or αSN (□) (6 to 50 μM). There were significant correlations between % TLR-4 in rafts and TNF for PrP82-146, Pearson’s coefficient = 0.88, Aβ1–42, Pearson’s coefficient = 0.78 and αSN Pearson’s coefficient = 0.86. (D) There was a significant correlation between the % TLR-4 in rafts of RAW 264 cells incubated with lipopolysaccharide (LPS) (0.75 to 50 ng/ml) and TNF produced, Pearson’s coefficient = 0.887.