Figure 5
From: Glimepiride reduces CD14 expression and cytokine secretion from macrophages
Glimepiride reduces cytokine secretion from microglial cells incubated with PrP82-146 or Aβ 1–42 . (A) The concentrations of TNF produced by microglial cells pre-treated for 1 hour with control medium (■), 5 μM glimepiride (□) or 5 μM glipizide (striped bars) and incubated with control medium, 50 μM PrP82-146 or 50 μM Aβ1–42 as shown. Values are means ± SD from triplicate experiments performed 3 times, n = 9. *TNF significantly less than control cells incubated with peptides. (B) The concentrations of TNF produced by microglial cells pre-treated with control medium (●) or 5 μM glimepiride for 1 hour (□), or pre-treated with 5 μM glimepirde for 1 hour and washed 3 times with PBS (striped bars) and incubated with 50 μM PrP82-146 or 50 μM Aβ1–42 as shown. Values are means ± SD from triplicate experiments performed 3 times, n = 9. *TNF significantly less than control cells incubated with peptides.