Figure 8
From: Glimepiride reduces CD14 expression and cytokine secretion from macrophages
PI-PLC digestion reduced cytokine secretion from RAW 264 cells. (A) The concentrations of TNF produced by RAW 264 cells pre-treated with control medium (□) or PI-PLC (■) and incubated with 50 μM PrP82-146, Aβ1–42 or αSN. Values are means ± SD from triplicate experiments performed 4 times, n = 12. *TNF significantly less than control cells incubated with peptides. (B) The concentrations of IL-1β produced by RAW 264 cells pre-treated with control medium (□) or PI-PLC (■) and incubated with 50 μM PrP82-146, Aβ1–42 or αSN. Values are means ± SD from duplicate experiments performed 4 times, n = 8. *IL-1β significantly less than control cells incubated with peptides. (C) The concentrations of IL-6 produced by RAW 264 cells pre-treated with control medium (□) or PI-PLC (■) and incubated with 50 μM PrP82-146, Aβ1–42 or αSN. Values are means ± SD from duplicate experiments performed 4 times, n = 8. *IL-6 significantly less than control cells incubated with peptides. *IL-6 significantly less than control cells incubated with peptides.