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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Inhibitory effect of ent-Sauchinone on amyloidogenesis via inhibition of STAT3-mediated NF-κB activation in cultured astrocytes and microglial BV-2 cells

Figure 2

Effect of ent -Sauchinone on LPS-induced ROS and NO release, protein expressions of iNOS and COX-2, and in astrocytes and microglial BV-2 cells. Cells were treated with 1 μg/ml of LPS alone, or with LPS plus different concentrations (1, 5, and 10 μM) of ent-Sauchinone, at 37°C for 24 hours. Intracellular ROS levels were determined by measuring DCF fluorescence (A and B). NO level was determined by Griess reaction, as described in Materials and Methods, in supernatants from astrocytes (C) and microglial BV-2 cells (D). Astrocytes (E) and microglial BV-2 cells (F) were treated with 1 μg/ml of LPS alone, or with LPS plus different concentrations (1, 5, and 10 μM) of ent-Sauchinone, at 37°C for 24 hours. Equal amounts of total protein (40 μg/lane) were subjected to 10% SDS-PAGE, and the expression of iNOS and COX-2 were detected by western blotting using specific antibodies. β-Actin protein was used here as an internal control. *indicates significantly different from the control group (P <0.05.. #indicates significantly different from the LPS-treated group (P <0.05.. Values below or above of each figures mean quantified relative expression of the proteins.

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