Figure 5

Cytokine production in response to inflammatory stimuli is enhanced in A20 deficient primary astrocytes. (A) TNF and (B) IL-6 levels, measured by ELISA, in cell culture supernatant from A20 wild type (WT), heterozygous (HT) and knockout (KO) mouse primary astrocytes following 24 hour stimulation with LPS (10 μg/mL). (C) IL-6 levels measured by ELISA, in cell culture supernatant from WT, HT and KO mouse primary astrocytes following 24 hours stimulation with TNF (100 UI/mL). NS: non-stimulated cells. Data represent mean ± SEM of primary astrocytes isolated from littermate pups (WT n = 2; HT n = 8 to 10, KO n = 3 to 4). *P < 0.05, **P < 0.01.