Reduced central nervous system demyelination, axonal damage and inflammatory cell infiltration and cytokine expression in mepazine-treated mice. Histopathology on spinal cords of vehicle-treated (n = 5) or mepazine-treated (n = 6) mice. (A) Demyelination (blue) and axon damage (APP, brown) were assessed. (B) Stainings for infiltrating T cells (CD3, brown) and macrophages (Mac-3, brown) by immunohistochemistry. Scale bars, 500 μm (top panel), 200 μm (lower panel). Expression of Th1 (C), Th2 (D), Th17 (E) and Treg-linked (F) factors in spinal cord of acceptor mice receiving splenocytes from vehicle- or mepazine-treated mice, 28 days post-splenocyte transfer. Data are depicted as mean ± SEM *P <0.05.