Figure 1

Analysis of mammalian target of rapamycin (mTOR) phosphorylation during microglial activation. Whole-cell lysates were prepared from microglial cells activated by control-conditioned medium (C-CM) (A) or CM from glioma cells activated with lipopolysaccharide (LPS)/IFNγ (LI-CM) (B). Equal amounts of proteins were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR), upper gel and were subsequently probed for β-actin, lower gel. Quantitation of densitometry where p-mTOR values are reflected relative to those for β-actin. Data are expressed as means ± standard error of the mean of n = 1 replicate for each group, each assayed in triplicate: representative of two different experiments. Data were analyzed by one-way analysis of variance followed by Bonferroni post hoc test. ***P <0.001 versus control.