Figure 2
From: The mTOR kinase inhibitors polarize glioma-activated microglia to express a M1 phenotype
Effects of the conditioned media on raptor and rictor expression. Total cytosolic RNA was prepared from control, or microglial cells treated with control-conditioned medium (C-CM) or CM from glioma cells activated with lipopolysaccharide (LPS)/IFNγ (LI-CM) for different times, and used for real time (Q)-PCR analysis of raptor (A) and rictor (B) expression. Data are expressed as fold change versus control, taken as calibrator for comparative quantitation analysis of mRNA levels. Each sample was measured in triplicate and the experiment was repeated twice with similar results. Data are means ± standard error of the mean, and were analyzed by two-way analysis of variance followed by Bonferroni post hoc test.