Effects of mammalian target of rapamycin (mTOR) inhibitors on M2 markers in microglia stimulated by both control-conditioned medium (C-CM) and LI-CM. Cells were treated with control-conditioned medium (C-CM) or CM from glioma cells activated with lipopolysaccharide (LPS)/IFNγ (LI-CM) and mTOR inhibitors. Total cytosolic RNA was prepared from control, or microglial cells treated with C-CM alone or with mTOR inhibitors for 4 h (A) and with LI-CM alone or with mTOR inhibitors for 4 h (B) or 24 h (C), and used for real time (Q)-PCR analysis of IL-10 (A-B) and arginase (ARG)1 (C) expression. Data are expressed as fold change versus control, taken as calibrator for comparative quantitation analysis of mRNA levels. Each sample was measured in triplicate and the experiment was repeated three times with similar results. Data are means ± standard error of the mean, and were analyzed by one-way analysis of variance followed by Bonferroni post hoc test. ***P <0.001 versus control and °°°P <0.001, °P <0.05, versus C-CM; ***P <0.001 versus control and °°P <0.01 versus LI-CM.