Figure 6
From: The mTOR kinase inhibitors polarize glioma-activated microglia to express a M1 phenotype
Effects of mammalian target of rapamycin (mTOR) inhibitors on microglial proliferation. Microglia cells were treated for 48 h with control-conditioned medium (C-CM) (A) or CM from glioma cells activated with lipopolysaccharide (LPS)/IFNγ (LI-CM) (B) and mTOR inhibitors. Effects on cell viability were assessed by the MTS reduction assay, while the effects on cell proliferation were assessed by 5-bromo-2-deoxyuridine (BrDU) incorporation. Data were analyzed by one-way analysis of variance followed by Bonferroni post hoc test.