Table 1 Primer sets used for Q-PCR analysis
From: The mTOR kinase inhibitors polarize glioma-activated microglia to express a M1 phenotype
Genes | Forward primers | Reverse primers | Product length (Base pair) |
|---|---|---|---|
α-TUB | CCC TCG CCA TGG TAA ATA CAT | ACT GGA TGG TAC GCT TGG TCT | 110 |
iNOS | CTG CAT GGA ACA GTA TAA GGC AAA C | CAG ACA GTT TCT GGT CGA TGT CAT GA | 230 |
IL-10 | CAG CTG CGA CGC TGT CAT CGA | GCA GTC CAG TAG ATG CCG GGT G | 198 |
ARG1 | TGC CCT CTG TCT TTT AGG GC | CCT CGA GGC TGT CCC TTA GA | 165 |
Raptor | GCA GGC CTG GGA CCT TGC TG | CGA CAG GGC CAA GCT CAC CG | 271 |
Rictor | AGC AGT GAT CCG AAA GGA GGG AAA | TAC TTG GAG TGC TGC CAG TGT CTT | 199 |