Transient global cerebral schemia decreases fractalkine/CX3CR1 Expression. (A) Decreased fractalkine expression was detected in the hippocampus of ischemic rats in comparison to the sham animals. Upper panel shows representative western blot. (B) Real-time polymerase chain reaction was used to validate the efficacy of siRNA delivery by measuring CX3CR1 mRNA in the hippocampus. Both ischemia and sham rats that received the siRNA showed significant decrease in CX3CR1 gene expression compared to the ischemia control and sham control groups, respectively. But ischemic control rats also showed decrease CX3CR1 gene expression when compared to the sham control animals. (C) Similar pattern of expression was seen when CX3CR1 protein was evaluated as seen in both the bar graph and representative western blots (upper panel). Legends: 1: ischemia/siRNA; 2: ischemia/control; 3: sham/siRNA; 4: sham/control. *P <0.5 and **P <0.01.