Interferon regulatory factor (IRF) 3 is critical for the development of experimental autoimmune encephalomyelitis

Table 2 Incidence of adoptively transferred experimental autoimmune encephalomyelitis with CD4 ⁺ cells from irf3 ^−/− and wild-type donors ^a

Experiment	WT to WT	irf3^−/−to WT	WT to irf3 ^−/−
1	6/6 (100%)	0/4 (0%)	0/6 (0%)
2	7/7 (100%)	0/4 (0%)	–
3	4/5 (80%)	0/5 (0%)	–
4	3/3 (100%)	–	0/5 (0%)
Total	20/21 (95%)	0/13 (0%)	0/11 (0%)

^aWild-type (WT) and interferon regulatory factor 3–knockout (irf3 ^−/−) mice were immunised with myelin oligodendrocyte glycoprotein (MOG_35–55) in complete Freund’s adjuvant medium. After 10 days, cells from spleens and lymph nodes were reactivated with MOG_35–55 in the presence of interleukin-23 for 3 days. Cells were transferred intravenously into naive WT or irf3 ^−/− recipient mice that received Bordetella pertussis toxin on day 0 and day 2 after cell transfer. Mice were scored daily for clinical signs of disease. Data represent disease incidence in each group in two to four independent experiments, and headings describe donor-to-recipient transfers.

ISSN: 1742-2094