Figure 4

Myelin basic protein and neurofilament expression is significantly decreased in transient systemic hypoxia-ischemia pups on postnatal day 28. (A) Following transient systemic hypoxia-ischemia (TSHI) on embryonic day 18, western blot analyses at postnatal day 28 reveal protein expression of myelin basic protein (MBP), phosphoneurofilament (pNF), and neurofilament (NF) is significantly altered in microdissected white matter. (B) MBP expression is significantly decreased in microdissected white matter from TSHI pups compared to sham levels (n = 3-10, **P < 0.01). (C) Similarly, the ratio of pNF/NF is significantly decreased in TSHI pups compared to shams, indicative of axonal dysfunction (n = 4-13, ***P < 0.001). LPS, lipopolysaccharide.