Figure 5

CCL2 hyperpolarizes serotonergic dorsal and median raphe neurons in mouse midbrain slices. (A) Example of recordings of the membrane potential of a presumed serotonergic neuron from the raphe nucleus recorded in current-clamp in response to increasing current pulses from −20 pA to 70 pA with an increment of 10 pA. (B) Spontaneous action potential discharge of a presumed sertonergic neuron recorded in current clamp and its response to the application of serotonin in the bath (5-HT; as indicated by the bar). (C) Graph presenting the instantaneous frequency of action potential discharge of a presumed serotonergic neuron recorded extracellularly in loose patch. The inset presents an example of recording obtained. (D) Spontaneous action potential discharge of a presumed serotonergic neuron recorded in current clamp and its response to the application of 1 nM CCL2 in the bath (as indicated by the bar).