Figure 2

Distribution of Iba-1+ cells in the retinal whole-mounts after 15 days of unilateral laser-induced OHT. Iba-1 immunostaining. Retinal whole-mount. The pressure produced by the cover slip on the whole-mount caused a retinal-like section effect on one edge of the tissue that revealed that: in naïve eyes (A) Iba-1+ cells were distributed in the NFL-GCL, IPL and OPL. Somas and processes of ramified Iba-1+ cells ran parallel to the retinal surface. In contralateral eyes (B) somas (blank arrowhead) and processes (arrow) displaced to and extended to the INL and ONL, respectively. In the OPL the long processes of Iba-1+ cells reached the OS (arrowhead). In OHT eyes (C) the features observed in contralateral eyes (B) were more intense. (INL: inner nuclear layer; IPL inner plexiform layer; NFL-GCL: nerve fiber layer-ganglion cell layer; OHT: ocular hypertension; ONL: outer nuclear layer; OPL: outer plexiform layer; OS: photoreceptor outer segment).