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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Microglia in mouse retina contralateral to experimental glaucoma exhibit multiple signs of activation in all retinal layers

Figure 5

CD68 expression in the NFL-GCL after 15 days of unilateral laser-induced OHT. Double immunostaining: Iba-1/CD68. Retinal whole-mount. In contralateral eyes (A, B) CD68 immunoreaction was detected only in some ameboid Iba-1+ cells which exhibited a patchy staining pattern (blank arrowhead). In the retinal whole-mount of OHT eyes (C-H), the greater CD68 immunoreaction was observed in this retinal layer. This was because, in addition to the CD68 immunoreactivity of ameboid Iba-1+ cells (blank arrowhead in C, D), rounded Iba-1+ cells (arrow in C-F) and some rod-like cells (blank arrow in G, H) had intense CD68+ cytoplasmic staining. Rounded Iba-1+ CD68+ cells were adjacent to the retinal vessels (C, D, F), being located mainly close to the optic disc (E) and in the periphery of the retina. In some instances, the processes of ramified Iba-1+ cell surrounded the rounded Iba-1+ cells (arrowhead in C) (NFL-GCL: nerve fiber layer-ganglion cell layer; OD: optic disc; OHT: ocular hypertension; v: retinal blood vessel).

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