Figure 7

Iba-1+ cells in the OPL after 15 days of unilateral laser-induced OHT. Iba-1 immunostaining. Retinal whole-mount. In the OPL only ramified Iba-1+ cells were observed. In comparison with naïve eyes (A-C), ramified Iba-1+ cells in the OPL of contralateral and OHT eyes formed a denser cellular mosaic (D, E, G, H). Iba-1+ cells in this layer exhibited morphological signs of cell activation (process retraction and increased secondary and superior order processes) that were more pronounced in OHT eyes (G-I) than in contralateral eyes (D-F). The cut-view analysis in the YZ (sagittal) and XY (coronal) plane demonstrate long processes (arrow) extending from the soma and running across the ONL into the OS (C, F, I). These long processes were more frequently observed in contralateral (F) and OHT eyes (I) than in naïve eyes (C). (OHT: ocular hypertension; ONL: Outer nuclear layer; OPL: outer plexiform layer, OS photoreceptor outer segment).