Figure 6

Reverse-transcriptase quantitative PCR ( RT-qPCR) demonstrates the level of inflammatory cytokines by BV2 microglia 6 hrs after lipopolysaccharide (LPS) stimulation. The values have been normalised to three housekeeping genes (Hmbs, Pgk1 and Psmb2). TNF, MCP-1, IL-1b, IL-6 and IL-12b were upregulated in stimulated BV2 microglia for three-dimensional (3D) cultures. Data are mean ± SEM from three independent experiments. *P < .05, **P < .01, ***P < .001, ****P < .0001; Welch’s t test.