siRNA-mediated downregulation of CD44 expression in BV2 cells. (A) Representative immunofluorescence images of CD44 (green) in control BV2 cells, CD44-siRNA-BV2 cells, and control-siRNA-BV2 cells. Nuclei are stained with DAPI (blue). Magnification × 400. (B) Western blot assay was performed to determine CD44 protein expression in extracts of control BV2 cells, CD44-siRNA-BV2 cells, and control-siRNA-BV2 cells. GAPDH was used as a loading control. Abbreviations: CD44-siRNA-BV2, BV2 cells transfected with CD44 siRNA; control-siRNA-BV2, BV2 cells transfected with control siRNA, DAPI, 4', 6-diamidino-2-phenylindole.