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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Mesenchymal stem cells inhibit lipopolysaccharide-induced inflammatory responses of BV2 microglial cells through TSG-6

Figure 4

siRNA-mediated downregulation of CD44 expression in BV2 cells. (A) Representative immunofluorescence images of CD44 (green) in control BV2 cells, CD44-siRNA-BV2 cells, and control-siRNA-BV2 cells. Nuclei are stained with DAPI (blue). Magnification × 400. (B) Western blot assay was performed to determine CD44 protein expression in extracts of control BV2 cells, CD44-siRNA-BV2 cells, and control-siRNA-BV2 cells. GAPDH was used as a loading control. Abbreviations: CD44-siRNA-BV2, BV2 cells transfected with CD44 siRNA; control-siRNA-BV2, BV2 cells transfected with control siRNA, DAPI, 4', 6-diamidino-2-phenylindole.

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