Continuous perichiasmal infusion of neuromyelitis optica immunoglobulin G antibody and complement produces retinal ganglion cells loss. (A) Retrograde labeling of retinal ganglion cells (RGCs) by injection of FluoroGold in the superior colliculus. After 7 days, neuromyelitis optica immunoglobulin G antibody (NMO-IgG) and complement were infused near the optic chiasm by minipump. Retinas were collected 7 days later. (B) Micrographs showing RGC loss. (C) Quantification of RGC number per 0.06 mm2 microscope field (mean ± SEM, n = 4, *P < 0.001). AQP4, aquaporin-4; HC, human complement; (ON, optic nerve).