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Figure 3 | Journal of Neuroinflammation

Figure 3

From: NitroDIGE analysis reveals inhibition of protein S-nitrosylation by epigallocatechin gallates in lipopolysaccharide-stimulated microglial cells

Figure 3

LPS-induced protein S-nitrosylation in BV-2 cells. (A) LPS-induced NO production. BV-2 cells were treated or untreated with 100 ng/mL LPS for 12, 16, or 20 hours. A Griess assay indicated NO production was elevated in the conditioned medium as the LPS incubation time increased. (B) NitroDIGE analysis of protein S-nitrosylation in BV-2 cells treated with LPS for 20 hours. Compared to untreated control, more green spots were seen from LPS-treated sample on the 2-DE gel. (C) Quantitative analysis revealed a total of 16 spots with significant S-nitrosylation level changes (fold change >1.3, P <0.05), from which 13 unique proteins were identified (Additional file 3: Table S2).

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Journal of Neuroinflammation

ISSN: 1742-2094