Figure 3

iNOS, gp91, and p22 expression in the trigeminal ganglia of the infected mice and NO production in the infected peritoneal macrophages. Mice were infected intranasally with 10⁶ p.f.u. of HSV-1 and were euthanized 5 days after the infection. The trigeminal ganglia were collected, the RNA extracted, reverse transcribed in cDNA and real-time PCR for the expression of (A) gp91^phox, (B) p22^phox, and (C) iNOS gene were performed. (D) Macrophages derived from the C57BL/6 mice or the indicated knockout mice were infected with HSV-1 (MOI of 10, five wells/group) (black bars) or were non-infected (NI, white bars), and the levels of nitric oxide (NO) were determined in the supernatants, 24 h after stimulation, by the Griess reaction assay. The bars represent the S.E.M. (*P <0.05, when comparing the infected macrophages to the respective non-infected macrophages; #P <0.05 or ##P <0.01 when comparing the C57BL/6 infected macrophages with the knockout infected macrophages. This experiment is representative of two analyses.