Figure 4
NO production by F4/80 + cells in the trigeminal ganglia of the infected mice. C57BL/6 (A, B, C, D) or TLR2/9−/− (E, F, G, H) mice were either inoculated with 106 p.f.u. of HSV-1 or with PBS (uninfected) via the intranasal route; on the fifth d.p.i., the trigeminal ganglia were collected and processed for analysis. (A) anti-iNOS Ab (green), C57BL/6 uninfected (400×); (B) anti-iNOS Ab (green), C57BL/6 infected (400×); (C) anti-iNOS Ab (red) plus anti-F4/80 Ab (green), C57BL/6 uninfected (100×); (D) anti-iNOS Ab (red) plus anti-F4/80 Ab (green), C57BL/6 infected, (200×); (E) anti-iNOS Ab (green), TLR2/9−/− uninfected, (100×); (F) anti-iNOS Ab (green), TLR2/9−/− infected (400×); (G) anti-iNOS Ab (red) plus anti-F4/80 Ab (green), TLR2/9−/− uninfected (400×); (H) anti-iNOS Ab (red) plus anti-F4/80 Ab (green), TLR2/9−/− infected (400×). All of the nuclei were stained with Hoechst (blue). Thin arrows (B, insert in B, F, insert in F) indicate stained iNOS cells (green); block arrows (D) indicate staining only for F4/80 (green) and thin arrows (D) indicate double staining for F4/80 and iNOS (brown). Short arrows (H) indicate staining for F4/80 (green), and long thin arrows (H) indicate stained nuclei (blue) characteristic of polymorphonuclear leukocytes.