Table 2 Antibodies used for FACS analysis
From: Macrophages migrate in an activation-dependent manner to chemokines involved in neuroinflammation
Antigen | Species and isotype | Clone | Dilution | Source |
|---|---|---|---|---|
CD31 | mIgG1 | JC70A | 1:100 | Dako, NL |
VLA-4 | mIgG1 | HP2/1 | 1:100 | Millipore, NL |
CCR2 | rbIgG | E68 | 1:50 | Abcam, UK |
CXCR3 | mIgG1 | 1C6 | 1:50 | BD, NL |
CXCR4 | mIgG1 | 12G5 | 1:50 | BD, NL |
CCR5 | mIgG1 | 2D7 | 1:50 | BD, NL |
Isotype control | mIgG1 | - | 0.2 μg/mL | Serotec, UK |