Figure 4

Suppressed proliferation of CD45 ^low /CD11b ⁺ /Ly6G ^- and CD45 ^hi /CD11b ⁺ /Ly6G ^- cells in the ipsilateral hemisphere of CX3CR1 ^-/- mice after middle cerebral artery occlusion. (A) Flow cytometry analysis of proliferation of Ly6G^– gated CD45^low/CD11b⁺ (microglia) and CD45^hi/CD11b⁺ (macrophages/activated microglia) population with 5-bromo-2-deoxyuridine (BrdU) incorporation in the ipsilateral hemisphere of wild-type (WT) and CX3CR1^-/- mice 72 hours after middle cerebral artery occlusion (MCAO), compared to their contralateral (unlesioned) hemisphere controls. CD45^hi/CD11b⁺/Ly6G^- cell proliferation was not shown in the contralateral due to their very low to undetectable presence. (B) Graph presents quantification of microglia/macrophage proliferation measured by flow cytometry. Data indicate a marked reduction in CD45^low/CD11b⁺/Ly6G^- cell proliferation and a modest but significant reduction in CD45^hi/CD11b⁺/Ly6G^- cell proliferation in the ipsilateral hemisphere of CX3CR1^-/- mice compared to WT mice 72 hours after MCAO. P < 0.0001 for genotype and localization, P = 0.0017 for interaction by two-way analysis of variance. *P < 0.05, **P < 0.01 by Bonferroni post-hoc tests. n = 4 per group.