Suppressed proliferation of CD45
cells in the ipsilateral hemisphere of CX3CR1
mice after middle cerebral artery occlusion. (A) Flow cytometry analysis of proliferation of Ly6G– gated CD45low/CD11b+ (microglia) and CD45hi/CD11b+ (macrophages/activated microglia) population with 5-bromo-2-deoxyuridine (BrdU) incorporation in the ipsilateral hemisphere of wild-type (WT) and CX3CR1-/- mice 72 hours after middle cerebral artery occlusion (MCAO), compared to their contralateral (unlesioned) hemisphere controls. CD45hi/CD11b+/Ly6G- cell proliferation was not shown in the contralateral due to their very low to undetectable presence. (B) Graph presents quantification of microglia/macrophage proliferation measured by flow cytometry. Data indicate a marked reduction in CD45low/CD11b+/Ly6G- cell proliferation and a modest but significant reduction in CD45hi/CD11b+/Ly6G- cell proliferation in the ipsilateral hemisphere of CX3CR1-/- mice compared to WT mice 72 hours after MCAO. P < 0.0001 for genotype and localization, P = 0.0017 for interaction by two-way analysis of variance. *P < 0.05, **P < 0.01 by Bonferroni post-hoc tests. n = 4 per group.