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Figure 6 | Journal of Neuroinflammation

Figure 6

From: CX3CR1 deficiency suppresses activation and neurotoxicity of microglia/macrophage in experimental ischemic stroke

Figure 6

CX3CR1 deficiency impairs inflammatory signaling in microglia and macrophage in ischemic brain. (A) The amounts of IL-1β, IL-6, and TNF-α in brain homogenates from wild-type (WT) and CX3CR1-/- mice 72 hours after middle cerebral artery occlusion (MCAO) were measured with ELISA. IL-1β: P = 0.0018 for genotype, P = 0.0002 for localization, and P = 0.0052 for interaction by two-way analysis of variance; IL-6: P = 0.0010 for genotype, P < 0.0001 for localization, and P = 0.0058 for interaction by two-way analysis of variance; TNF-α: P < 0.0001 for genotype, P = 0.0240 for localization, and P = 0.0063 for interaction by two-way analysis of variance. *P < 0.05, **P < 0.01 by Bonferroni post-hoc tests. n = 4 per group. (B) IL-1β, IL-6, and TNF-α expression were analyzed by flow cytometry within the CD11b+Ly6G gate. Representative histograms show IL-1β, IL-6, and TNF-α expression in the contralateral (blue) and ipsilateral (red) hemispheres of CX3CR1-/- and WT mice at 72 hours after MCAO. Mean fluorescent intensity is indicated within each representative histogram. (C) The number of IL-1β, IL-6, and TNF-α-producing CD11b+Ly6G cells was quantified from ischemic brain of CX3CR1-/- and WT mice at 72 hours after MCAO with flowcytometry. IL-1β+/CD11b+/Ly6G: P = 0.0030 for genotype, P = 0.0002 for localization, and P = 0.0052 for interaction by two-way analysis of variance; IL-6+/CD11b+/Ly6G: P = 0.0030 for genotype, P = 0.0002 for localization, and P = 0.0052 for interaction by two-way analysis of variance; TNF-α+/CD11b+/Ly6G: P = 0.0386 for genotype, P = 0.0332 for localization, and P = 0.0173 for interaction by two-way analysis of variance. *P < 0.05 by Bonferroni post-hoc tests. n = 4 per group.

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