Effect of Ilantide on interleukin 1β-induced rat islet cell apoptosis. Rat pancreatic islets were preincubated for 30 minutes in the absence or presence of various concentrations of anakinra (A), Ilantide-m (B) or Ilantide-t (C) and then exposed to 8.68 nM interleukin 1β (IL-1β) for 24 hours. Cytokine-induced apoptosis was determined by cell death detection enzyme-linked immunosorbent assay. Apoptosis rates are presented as fold induction compared with untreated control islets (white bars). The data from four independent experiments are presented as mean ± SEM. *P < 0.05 and **P < 0.01 compared with cultures treated with IL-1β alone (one-way analysis of variance followed by Dunnett’s post hoc test).