S. aureus- activated Müller glia secrete antimicrobial peptides (AMPs) into culture media. MIO-M1 cells were infected with S. aureus (SA) for the indicated time points. The secretion of AMPs into the culture supernatant was detected via dot-blot (A), and the intensities of the dots were quantitated by densitometric analysis and presented as fold-changes, using a value of 1 for the control samples (B). Statistical analysis was performed using one-way ANOVA (*P < 0.05; **P < 0.005), for comparisons of control versus stimulated cells over the time. Data points and bars represent mean ± SD of triplicates from three independent experiments.