Activated Müller glia-conditioned media possesses antibacterial properties. MIO-M1 cells were challenged with S. aureus (SA). Following a four hour incubation, the culture supernatant was subjected to a serial dilution and plating for bacterial colony forming unit (CFU) enumeration. Bacteria were also grown in identical culture conditions without Müller cell contact to be used as control (A). In another experiment, suspended MIO-M1 cells (8 x 105) were co-cultured with a 4 x 103 CFU of SA (200:1 ratio) in Hank’s balanced salt solution (HBSS) for one hour at 37°C. The control for this experiment includes bacteria grown separately (without cells). Following incubation, the tubes were sonicated and bacterial CFU was estimated via dilution plating (B). Statistical analysis was performed using a student’s t-test (*P < 0.05). The data represent a cumulative of three independent experiments performed in triplicates.