Validation of the stress model. (A) Body weight gain (in grams). *P < 0.05. (B) Adrenal gland weight (in milligrams; bars) and ratio between adrenal gland weight and body weight gain (scatterplot and line). **P < 0.01, #
P < 0.01 (for adrenal gland weight/body weight gain ratio). (C) Serum corticosterone (percentage of control animals). *P < 0.01 compared with control, a
P < 0.01 compared with previous time point (S1d to S10d indicates days subjected to variate stress). Statistical significance was calculated by using Student’s t-test to compare data before (C) and after 10 days (S10d) of variate stress. Data were derived from one-way analysis of variance followed by the least significant difference (LSD) post hoc test for multiple range comparisons.