Time-dependent uptake of apoptotic bodies by MECs. (A) Ben-Men-I cells were incubated with CFSE-labeled apoptotic U-937 cells for 4, 12, or 24 h at a MEC:apoptotic body ratio of 1:5. CFSE-fluorescence was analyzed using flow cytometry. The bar graphs (B) depict the percentage of CFSE-positive MECs (left graph) and the mean fluorescence intensity (MFI - right graph) of three independent experiments with the error bars representing SD. Statistical significance was analyzed by one-way ANOVA. Pair-wise Student’s t-test revealed P <0.001 adjusted according to Holm for all comparisons (marked ***) except where noted otherwise (n.s. P >0.05).