Uptake of apoptotic neuronal cells by MECs. (A) CellTrace Violet-labeled Ben-Men-I cells were incubated with CFSE-labeled apoptotic SH-SY5Y cells for 4 (middle panel) and 24 (right panel) h at a MEC:apoptotic body ratio of 1:5. CellTrace violet and CFSE-fluorescence was analyzed using flow cytometry. MECs not incubated with apoptotic bodies (left panel) served as control. (B) CFSE-labeled neuroblastoma-derived apoptotic bodies were incubated with CellTrace Violet-labeled Ben-Men-I cells at a ratio of 1:0, 1:1, 1:5, 1:10 for 24 h and cellular uptake was analyzed by flow cytometry. Shown is the average of three independent experiments with error bars depicting SD. Statistical significance was analyzed by one-way ANOVA. Pair-wise Student’s t-test revealed P <0.001 adjusted according to Holm for all comparisons (marked ***).