RM-1 cell inoculation increases pNFκB expression in spinal astrocytes. (A,B) Western blot shows that tumor cell inoculation increased pNFκB expression in the spinal cord at 7 days, 14 days, and 21 days. *P <0.05, **P <0.01 vs. naive. One-way ANOVA followed by Newman-Keuls test. n = 4 mice per group. (C, D) Immunostaining shows that pNFκB was expressed in the superficial dorsal horn in sham animals (C), increased at 7 days in inoculated animals (D). (E-G) Double staining showed pNFκB was colocalized with GFAP.