Figure 7
NFκB inhibitor attenuated RM-1 cell inoculation-induced pain hypersensitivity and upregulation of CXCL1 in the spinal cord. (A, B) Intrathecal injection of NFκB inhibitor, BAY11-7082 at the dose of 0.4 μg had no effect on mechanical allodynia or heat hyperalgesia, whereas at the dose of 4 μg attenuated mechanical allodynia (A) and heat hyperalgesia (B) at 1 h and 3 h. *P <0.05, **P <0.01, ***P <0.001 vs. vehicle. One-way ANOVA followed by Newman-Keuls test. (C, D) Immunostaining of CXCL1 in the spinal cord in vehicle and BAY11-7082-treated animals. (E) The CXCL1-IF intensity was decreased by BAY11-7082. ***P <0.001 vs. vehicle. n = 4 mice per group.