Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin deficient mice

Niesman, Ingrid R; Schilling, Jan M; Shapiro, Lee A; Kellerhals, Sarah E; Bonds, Jacqueline A; Kleschevnikov, Alexander M; Cui, Weihua; Voong, April; Krajewski, Stan; Ali, Sameh S; Roth, David M; Patel, Hemal H; Patel, Piyush M; Head, Brian P

doi:10.1186/1742-2094-11-39

Journal of Neuroinflammation

Table 1 Multiplex array reveals brain changes in certain cytokines, chemokines, and growth factors after CCI

From: Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin deficient mice

Ipsilateral						Contralateral
	WT		Cav-I KO		Cav-3 KO		WT		Cav-1 KO		Cav-3 KO
	Sham	TBI	Sham	TBI	Sham	TBI	Sham	TBI	Sham	TBI	Sham	TBI
IL-1β	0.072 ± 0.004	0.83 ± 0.06*	0.06 ± 0.005	1.4 ± 0.3*	0.07 ± 0.007	*2.5 ± 0.4#**	0.08 ± 0.01	0.59 ± 0.07	0.06 ± 0.005	1.05 ± 0.13*	0.07 ± 0.01	*2.3 ± 0.2#^**
IL-2	011 ± 0.002	0.36 ± 0.03	0.11 ± 0.005	*0.7 ± 0.07#**	013 ± 0.004	0.96 ± 0.15#	0.11 ± 0.01	0.22 ± 0.05	0.12 ± 0.004	0.6 ± 0.1*	0.13 ± 0.01	*0.9 ± 0.1#^**
IL-6	0.04 ± 0.006	2.0 ± 0.4	0.03 ± 0.007	*30.9 ± 3.9#**	0.03 ± 0.003	*42.2 ± 7.1#**	0.03 ± 0.005	0.58 ± 0.06	0.02 ± 0.001	*14.2 ± 3.9#δ**	0.02 ± 0.005	*7.5 ± 1.3#**
IL-9	0.69 ± 0.03	9.6 ± 0.4*	0.6 ± 0.06	15.1 ± 1.5*	0.8 ± 0.09	*23.3 ± 3.3#^**	0.79 ± 0.19	7.4 ± 1.3*	0.8 ± 0.03	*13.6 ± 2.2#**	0.7 ± 0.06	*21.0 ± 1.5#^**
IL-10	0.034 ± 0.003	1.0 ± 0.08*	0.04 ± 0.003	1.5 ± 0.2*	0.06 ± 0.002	*2.0 ± 0.3#**	0.046 ± 0.012	0.62 ± 0.08*	0.04 ± 0.001	1.03 ± 0.17*	0.05 ± 0.01	*1.8 ± 0.1#^**
IL-I7	0.0009 ± 0.0003	0.22 ± 0.02	0.002 ± 0.0003	0.3 ± 0.04*	0.003 ± 0.0001	0.4 ± 0.1*	0.0014 ± 0.0005	0.13 ± 0.03*	0.001 ± 0.0002	*0.3 ± 0.05#**	0.003 ± 0.001	*0.4 ± 0.04#^**
KC	0054 ± 0.01	6.3 ± 1.2*	0.05 ± 0.006	*31.8 ± 3.1#**	0.05 ± 0.005	*49.1 ± 7.7#^**	0.027 ± 0.009	1.6 ± 0.2	0.02 ± 0.001	*9.5 ± 1.8#**	0.03 ± 0.01	*11.0 ± 2.5#**
MCP-1	0.051 ± 0.004	7.0 ± 2.2*	0.05 ± 0.009	*19.4 ± 1.5#**	0.05 ± 0.004	*39.4 ± 6.3#^**	0.042 ± 0.009	1.9 ± 0.2	0.04 ± 0.004	5.6 ± 1.2*	0.07 ± 0.01	*8.6 ± 1.9#**
MIP-1α	0.031 ± 0.004	2.0 ± 0.07	0.02 ± 0.008	6.7 ± 0.6	0.03 ± 0.003	*20.1 ± 4.8#^**	0.022 ± 0.006	1.6 ± 0.2*	0.01 ± 0.002	2.0 ± 0.6*	0.02 ± 0.01	2.4 ± 0.3*
GMCSF	NA	0.84 ± 0.2	NA	1.9 ± 0.6	0.2 ± 0.03	*3.4 ± 0.6#^**	0.14 ± 0.05	0.7 ± 0.2	0.11 ± 0.01	*2.0 ± 0.4#**	0.3 ± 0.04	*2.5 ± 0.3#**

Four hours post-CCI, contralateral and ipsilateral hemispheres were analyzed with the MAGPIX Cytokine Multiplex Assay for IL-1β, IL-2, IL-6, IL-9, IL-10, IL-17, KC (keratinocyte chemoattractant), monocyte chemoattractant protein 1 (MCP-1), macrophage inflammatory protein 1 alpha (MIP-1α), and granulocyte-macrophage colony-stimulating factor (GMCSF). Data (n = 6 mice/group) represent mean ± SEM. P < 0.05 or less were considered statistically significant. *P < 0.05 versus sham hemisphere, #P < 0.05 versus wild-type (WT) traumatic brain injury (TBI) hemisphere, ^P < 0.05 versus caveolin (Cav)-1 knock-out (KO) TBI hemisphere, δP < 0.05 versus Cav-3 KO TBI hemisphere. NA, not available (below detection).

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ISSN: 1742-2094

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