Extensive dysregulations of oligodendrocytic and astrocytic connexins are associated with disease progression in an amyotrophic lateral sclerosis mouse model

Table 1 Antibodies used for immunohistochemistry

Antigen	Type	Dilution	Antigen retrieval	Source
Astrocyte
Cx43	Rabbit polyclonal	1:1000	Not done	Abcam, Cambridge, UK
Cx30	Rabbit polyclonal	1:100	Autoclave, 10 mM citrate buffer	Sigma Aldrich, St Louis, USA
AQP4	Rabbit polyclonal	1:500	Not done	Santa Cruz Biotechnology, California, USA
GFAP	Rabbit polyclonal	1:1000	Not done	DakoCytomation, Glostrup, Denmark
EAAT2	Mouse monoclonal	1:50	Not done	Novocastra, Newcastle upon Tyne, UK
Oligodendrocyte or myelin
Cx32	Mouse monoclonal	1:200	Autoclave, 10 mM citrate buffer	Life Technologies, California, USA
Cx47	Mouse monoclonal	1:200	Autoclave, 10 mM citrate buffer	Life Technologies, California, USA
MOG	Rabbit polyclonal	1:1000	Not done	Sigma Aldrich, St Louis, USA
Nogo-A	Rabbit polyclonal	1:400	Not done	Santa Cruz Biotechnology, California, USA
CC1	Mouse monoclonal	1:1000	Autoclave, 10 mM citrate buffer	Abcam, Cambridge, UK
Microglia
Iba-1	Rabbit polyclonal	1:1000	Not done	Wako, Osaka, Japan
Neuron
NeuN	Mouse monoclonal	1:100	Not done	Merck Millipore, Darmstadt, Germany
Axon
Neurofilament	Mouse monoclonal	1:100	Not done	DakoCytomation, Glostrup, Denmark
Superoxide dismutase 1
SOD1	Rabbit polyclonal	1:100	Autoclave, 10 mM citrate buffer	Abcam, Cambridge, UK
Apoptotic cell
Cleaved caspase-3	Rabbit polyclonal	1:1000	Autoclave, 10 mM citrate buffer	Cell Signaling Technology, MA, USA

AQP4, aquaporin-4; Cx, connexin; EAAT2, excitatory amino acid transporter-2; GFAP, glial fibrillary acidic protein; Iba-1, ionized calcium-binding adapter molecule 1; MOG, myelin-oligodendrocyte glycoprotein; SOD1, superoxide dismutase 1.

ISSN: 1742-2094