Extensive dysregulations of oligodendrocytic and astrocytic connexins are associated with disease progression in an amyotrophic lateral sclerosis mouse model

Table 3 Summary of oligodendrocytic and astrocytic changes in the anterior horns of mSOD1-Tg mice

	12 weeks of age (presymptomatic stage)			18 weeks of age (disease-progressive stage)			20 weeks of age (end stage)
	Immunohistochemistry (n= 5)	qIB (n= 3)	qPCR (n= 3)	Immunohistochemistry ( n= 7)	qIB (n= 3)	qPCR (n= 3)	Immunohistochemistry (n= 5)	qIB (n= 5)	qPCR (n= 3)
Cx32	Unchanged	Unchanged	Unchanged	Decreased	Decreased	Unchanged	Decreased	Decreased	Decreased
Cx47	Unchanged	Unchanged	Unchanged	Decreased (internalized)	Unchanged	Decreased	Decreased (internalized)	Decreased	Unchanged
Cx30	Unchanged	Unchanged	Unchanged	Unchanged	Unchanged	Unchanged	Unchanged	Unchanged	Unchanged
Cx43	Unchanged	Unchanged	Increased	Increased	Unchanged	Unchanged	Increased	Unchanged	Increased
GFAP	Unchanged	Unchanged	Not done	Increased	Increased	Not done	Increased	Increased	Not done
EAAT2	Unchanged	Unchanged	Not done	Decreased (3/7)	Decreased	Not done	Decreased (2/5)	Decreased	Not done
MOG	Unchanged	Unchanged	Not done	Unchanged	Unchanged	Not done	Unchanged	Unchanged	Not done

Cx, connexin; EAAT2, excitatory amino acid transporter-2; GFAP, glial fibrillary acidic protein; MOG, myelin-oligodendrocyte glycoprotein; qIB, quantitative immunoblot; qPCR, quantitative real-time polymerase chain reaction.

ISSN: 1742-2094