Figure 4

Removal of type-1 IFN signalling confers a decreased pro-inflammatory response to OGD. M17 NC shRNA and IFNAR1 KD cells were subjected to 4.5 h of OGD and up to 24 h of reperfusion. Q-PCR was then performed detecting: (A) IL-1β, (B) IL-6, (C) TNFα, (D) IFNα and (E) IFNβ mRNA levels (*P <0.05, ***P <0.001, n = 5, unmatched two-way ANOVA, Bonferroni post-hoc test). Data are expressed as fold change in mRNA transcript levels in reference to no OGD control samples. Reperfusion times are indicated in hours on the x axis and graphical data are represented as mean ± SEM.