Figure 5

IFNAR1 dependant type-1 IFN signalling is detrimental to cellular viability in response to OGD. M17 cells either (A) over-expressing IFNAR1 or (B) with IFNAR1 knocked down were subjected to 4.5 h of OGD and 24 h of reperfusion and an MTT assay for cell viability was performed (*P <0.05, ***P <0.01, n = 4, unpaired two-tailed student’s t-test). Q-PCR was used to quantify IFNAR1 levels in M17 cells transiently transfected with (C) a pcDNA6.2/cEM-GFP-IFNAR1 over-expression plasmid or stably expressing (D) an IFNAR1 shRNA construct (n = 3). Graphical data are represented as mean ± SEM.