Figure 6

Ablation of type-1 IFN signalling, through removal of IFNAR1, decreases pro-apoptotic cleaved-caspase-3 levels following O GD. M17 NC shRNA and IFNAR1 KD cells were subjected to 4.5 h of OGD and up to 24 h of reperfusion. Cell lysates were analysed by western blot probed for cleaved and total caspase-3 levels (*P <0.05, n = 5, unmatched one-way ANOVA, Dunnett’s post-hoc test); β-actin was used as a loading control. For densitometry calculations cleaved caspase-3 intensity was measured in arbitrary units (A. U.) and normalised to the Caspase-3: β-actin intensity ratio. These values were then expressed as fold change to the relevant genotype control and are displayed as mean ± SEM. No statistical analysis was performed on the IFNAR1 KD blots as bands remained undetected across all time-points.