Figure 1

Immunohistochemical differentiation of anti-endothelial cell antibodies (AECA) and aquaporin-4 (AQP4)-IgG by double staining with patient serum and a commercial anti-human AQP4 antibody. While AECA (green) stain the endothelial cells, the AQP4 antibody (red) stains the astrocytic endfeet surrounding the microvasculature.