IVIg protects the 3xTg-AD mouse model of Alzheimer’s disease from memory deficit and Aβ pathology

Table 2 Levels of proteins in the parietotemporal cortex of 3xTg-AD mice

Biological function	Proteins	Age group	Fraction	NonTg ctrl	3xTg-AD ctrl	3xTg-AD IVIg	ANOVA Pvalue
Synaptic	Snap25	12 months	Membrane	1.58 ± 0.38	1.48 ± 0.4	1.46 ± 0.38	0.78
	PSD95	12 months	Membrane	1.31 ± 0.33	1.39 ± 0.31	1.39 ± 0.33	0.85
	Septin3	16 months	Cytosolic	1.16 ± 0.17	1.55 ± 0.38*	1.60 ± 0.42*	0.04
	Dynamin1	16 months	Membrane	1.56 ± 0.23	1.38 ± 0.15	1.50 ± 0.12	0.75
	Drebrin	16 months	Membrane	1.35 ± 0.24	1.57 ± 0.55	1.63 ± 0.41	0.36
	Synaptophysin	16 months	Membrane	6.73 ± 1.5	6.86 ± 1.13	6.82 ± 1.16	0.97
Others	VILIP-1	12 months	Cytosolic	3.01 ± 0.59	2.68 ± 0.42	2.58 ± 0.35	0.10
	VILIP-1	16 months	Cytosolic	1.74 ± 0.19	1.58 ± 0.31	1.58 ± 0.25*	0.01
	ADAM-10	12 months	Cytosolic	8.08 ± 0.45	7.38 ± 0.23	7.55 ± 0.39	0.43
	ADAM-10	16 months	Cytosolic	7.97 ± 0.51	8.27 ± 0.37	7.36 ± 0.24	0.27
	PAK	16 months	Cytosolic	8.05 ± 2.49	6.81 ± 0.71	7.50 ± 0.93	0.07

Western blot analyses. Equal loading of samples was determined by protein quantification and actin staining. Values are expressed as the mean ± SEM of RLU (n = 7 to 13 animals). Statistical analysis: one-way ANOVA followed with Tukey’s multiple comparison test. *P <0.05 versus NonTg ctrl; ^§ P <0.05 versus 3xTg-AD ctrl. 3xTg-AD, triple-transgenic mouse model of Alzheimer’s disease; ADAM-10, a disintegrin and metalloproteinase domain containing protein 10; ANOVA, analysis of variance; ctrl, control; IVIg, human intravenous immunoglobulin; NonTg, non-transgenic; PAK, p21-activated kinase; PSD95, postsynaptic density protein 95; RLU, relative luminescence unit; Snap25, synaptosomal-associated protein 25; VILIP-1, visinin-like protein-1.

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