Table 1 Antibodies and detection reagents for immunohistochemistry

Anti-Iba1

Rabbit polyclonal

1:500

2 hours, RT

Anti-iNOS

Mouse monoclonal

1:100

2 hours, RT

Anti-arginase1

Mouse monoclonal

1:100

O/N, 4°C

Anti-ubiquitin

Rabbit polyclonal

1:1000

O/N, RT

Anti-dephosphorylated neurofilament (SMI32)

Mouse monoclonal

1:2000

O/N, RT

*Anti-rabbit IgG, biotinylated

Goat polyclonal

1:500

1 hour, RT

*Anti-rabbit IgG, AlexaFluor^®594-conjugated

Goat polyclonal

1:1000

2 hours, RT

*Anti-mouse IgG, AlexaFluor^®488-conjugated

Goat polyclonal

1:1000

2 hours, RT

*MOM kit anti-mouse IgG, biotinylated

MOM kit

1:250

20 minutes, RT

Streptavidin, AlexaFluor^®488-conjugated

Streptavidin

1:500

1 hour, RT

Streptavidin, AlexaFluor^®594-conjugated

Streptavidin

1:500

2 hours, RT

Nuclear yellow

Nuclear dye

1:10000

20 minutes, RT