Figure 1

Comparison of microglia and infiltrating cells in the central nervous system during inflammation. Representative flow cytometry profiles of individual central nervous system suspensions prepared from control mice or mice with severe experimental autoimmune encephalomyelitis (EAE) showing staining for CD11c and CD45. Vertical line indicates isotype control. Cytometry profile shows increase of both microglia and infiltrating cells expressing CD11c (A). Flow cytometry analysis from six combined experiments shows a significant increase of CD11c⁺ microglia presented as a percentage from total microglia (B). CD11c⁺ microglia showed an increased expression of CD45, but the expression did not reach the level of CD45^high expressing cells (C). Quantitative real-time PCR analysis shows significantly lower expression of CCR2 in both population of microglia than in CD45^high CD11c⁺ cells (D). Data are presented as a means ± SEM of six individual experiments (n ≥ 13). ***P < 0.001.