Figure 4

Relative efficiency of central nervous system and spleen antigen presenting cells in stimulating proliferation of myelin-specific CD4 T cells. Proliferation of CD4 T cells sorted from B6 mice with severe experimental autoimmune encephalomyelitis (EAE). Splenic CD4⁺ T cells from myelin oligodendrocyte glycoprotein (MOG)_p35–55 immunised B6 mice with severe EAE were isolated using magnetic beads and cultured with antigen presenting cells (CD11c⁻ microglia, CD11c⁺ microglia, CD45^high CD11c⁺ cells and spleen-derived dendritic cells (spl. DC), with (black bars) or without (white bars) MOG_p35–55. Proliferation was measured by CFSE dilution assay. Data are presented as means ± SEM of three individual experiments (n ≥ 9 ). ns, Non-significant differences (P > 0.05).